The Ley lab studies atherosclerosis, the pathomechanism underlying most cardiovascular disease. We focus on the adaptive immune system. We work on the autoimmune aspect of atherosclerosis, mainly on the CD4 and CD8 T cell responses.
Klaus Ley, M.D.
Founding Co-Director, Immunology Center of Georgia (IMMCG)
Georgia Research Alliance Bradley Turner Eminent Scholar in Immunology
Professor, Department of Physiology
Medical College of Georgia at Augusta University
The lab uses human samples from patients with the disease we study: cardiovascular disease including coronary artery disease. Major methods we use:
Immunology Center of Georgia (IMMCG)
Health Sciences Campus
Georgia Cancer Center - M. Bert Storey Research Building
1410 Laney Walker Blvd, CN2119
706-446-0296
Anusha Bellapu
India
Michael Nakama, MS
United States of America
Pei Xiong Liew, PhD
Singapore
Mikhail Fomin, MSc
United States of America
Payel Roy, PhD
India
Mohammad Oliaeimotlagh, MD MSc
Iran
Smriti Parashar, PhD
India
Sunil Kumar, Ph.D.
India
Yan Wang, PhD
China
Megh Mehta
United States of America
Aderonke Fakayode
Nigeria
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PD-1 and CD73 on naive CD4+ T cells synergistically limit responses to selfNettersheim, F. S., Brunel, S., Sinkovits, R. S., Armstrong, S. S., Roy, P., Billitti, M., Kobiyama, K., Alimadadi, A., Bombin, S., Lu, L., Zoccheddu, M., Oliaeimotlagh, M., Benedict, C. A., Sette, A. & Ley, K., 2024, (Accepted/In press) In: Nature Immunology.Research output: Contribution to journal › Article › peer-review |
Olfr2-positive macrophages originate from monocytes proliferate in situ and present a pro-inflammatory foamy-like phenotypeSuthahar, S. S. A., Nettersheim, F. S., Alimadadi, A., Wang, E., Billitti, M., Resto-Trujillo, N., Roy, P., Hedrick, C., Ley, K. & Orecchioni, M., Sep 1 2024, In: Cardiovascular Research. 120, 13, p. 1577-1589 13 p.Research output: Contribution to journal › Article › peer-review |
Editorial: Adaptive immunity in atherosclerosisSlütter, B. & Ley, K., 2024, In: Frontiers in immunology. 15, 1440283.Research output: Contribution to journal › Editorial › peer-review |
Mild Hypoxia Accelerates Cerebral Cavernous Malformation Disease Through CX3CR1-CX3CL1 SignalingFrias-Anaya, E., Gallego-Gutierrez, H., Gongol, B., Weinsheimer, S., Lai, C. C., Orecchioni, M., Sriram, A., Bui, C. M., Nelsen, B., Hale, P., Pham, A., Shenkar, R., Debiasse, D., Lightle, R., Girard, R., Li, Y., Srinath, A., Daneman, R., Nudleman, E. & Sun, H. & 8 others, , Jun 1 2024, In: Arteriosclerosis, thrombosis, and vascular biology. 44, 6, p. 1246-1264 19 p.Research output: Contribution to journal › Article › peer-review |
Identification of apolipoprotein B–reactive CDR3 motifs allows tracking of atherosclerosis-related memory CD4+T cells in multiple donorsRoy, P., Suthahar, S. S. A., Makings, J. & Ley, K., 2024, In: Frontiers in immunology. 15, 1302031.Research output: Contribution to journal › Article › peer-review |
Immunotherapy for atherosclerosis by targeting pro-inflammatory T cellsKhan, A. & Ley, K., Jul 2024, In: Cell Research. 34, 7, p. 467-468 2 p.Research output: Contribution to journal › Article › peer-review |
Dysregulated cellular metabolism in atherosclerosis: mediators and therapeutic opportunitiesStroope, C., Nettersheim, F. S., Coon, B., Finney, A. C., Schwartz, M. A., Ley, K., Rom, O. & Yurdagul, A., Apr 2024, In: Nature Metabolism. 6, 4, p. 617-638 22 p.Research output: Contribution to journal › Review article › peer-review |
Immune profiling and tracking of two-dimensional transition metal dichalcogenides in cells and tissuesGazzi, A., Fusco, L., Orecchioni, M., Keshavan, S., Shin, Y., Grivel, J. C., Rinchai, D., Ahmed, E. I., Elhanani, O., Furesi, G., Rauner, M., Keren, L., Ley, K., Casiraghi, C., Bedognetti, D., Fadeel, B. & Delogu, L. G., Feb 2024, In: Nano Today. 54, 102084.Research output: Contribution to journal › Article › peer-review |
Single Cell High Dimensional Analysis of Human Peripheral Blood Mononuclear Cells Reveals Unique Intermediate Monocyte Subsets Associated with Sex Differences in Coronary Artery DiseaseChatterjee, N., Komaravolu, R. K., Durant, C. P., Wu, R., McSkimming, C., Drago, F., Kumar, S., Valentin-Guillama, G., Miller, Y. I., McNamara, C. A., Ley, K., Taylor, A., Alimadadi, A. & Hedrick, C. C., Mar 2024, In: International journal of molecular sciences. 25, 5, 2894.Research output: Contribution to journal › Article › peer-review |
Breaking tolerance: the autoimmune aspect of atherosclerosisKhan, A., Roy, P. & Ley, K., Sep 2024, In: Nature Reviews Immunology. 24, 9, p. 670-679 10 p.Research output: Contribution to journal › Article › peer-review |
Single-cell profiling of CD11c+ B cells in atherosclerosisPattarabanjird, T., Srikakulapu, P., Ransegnola, B., Marshall, M. A., Ghosheh, Y., Gulati, R., Durant, C., Drago, F., Taylor, A. M., Ley, K. & McNamara, C. A., 2023, In: Frontiers in immunology. 14, 1296668.Research output: Contribution to journal › Article › peer-review |
Single cell transcriptomics reveals recent CD8T cell receptor signaling in patients with coronary artery diseaseIqneibi, S., Saigusa, R., Khan, A., Oliaeimotlagh, M., Armstrong Suthahar, S. S., Kumar, S., Alimadadi, A., Durant, C. P., Ghosheh, Y., McNamara, C. A., Hedrick, C. C. & Ley, K., 2023, In: Frontiers in immunology. 14, 1239148.Research output: Contribution to journal › Article › peer-review |
Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosisKiss, M. G., Papac-Miličević, N., Porsch, F., Tsiantoulas, D., Hendrikx, T., Takaoka, M., Dinh, H. Q., Narzt, M. S., Göderle, L., Ozsvár-Kozma, M., Schuster, M., Fortelny, N., Hladik, A., Knapp, S., Gruber, F., Pickering, M. C., Bock, C., Swirski, F. K., Ley, K. & Zernecke, A. & 4 others, , Aug 8 2023, In: Immunity. 56, 8, p. 1809-1824.e10Research output: Contribution to journal › Article › peer-review |
Single-cell profiling reveals inflammatory polarization of human carotid versus femoral plaque leukocytesSlysz, J., Sinha, A., DeBerge, M., Singh, S., Avgousti, H., Lee, I., Glinton, K., Nagasaka, R., Dalal, P., Alexandria, S., Wai, C. M., Tellez, R., Vescovo, M., Sunderraj, A., Wang, X., Schipma, M., Sisk, R., Gulati, R., Vallejo, J. & Saigusa, R. & 8 others, , 2023, In: JCI Insight. 8, 17, e171359.Research output: Contribution to journal › Article › peer-review |
Mast cells must stickLyu, Q., Wang, Y. & Ley, K., Aug 2023, In: Journal of Leukocyte Biology. 114, 2, p. 109-110 2 p.Research output: Contribution to journal › Editorial › peer-review |
Author Correction: Human circulating CD24hi marginal zone B cells produce IgM targeting atherogenic antigens and confer protection from vascular disease (Nature Cardiovascular Research, (2023), 2, 11, (1003-1014), 10.1038/s44161-023-00356-1)Pattarabanjird, T., Nguyen, A. T., McSkimming, C., Dinh, H. Q., Marshall, M. A., Ghosheh, Y., Gulati, R., Durant, C., Vallejo, J., Saigusa, R., Drago, F., Guy, T. V., Premo, K., Taylor, A. M., Paul, S., Kundu, B., Berr, S., Gonen, A., Tsimikas, S. & Miller, Y. & 4 others, , Dec 2023, In: Nature Cardiovascular Research. 2, 12, p. 1328 1 p.Research output: Contribution to journal › Comment/debate › peer-review |
Chemokine Receptor Activation Enhances Memory B Cell Class Switching Linked to IgE Sensitization to Alpha Gal and Cardiovascular DiseasePattarabanjird, T., Wilson, J. M., Erickson, L. D., Workman, L. J., Qiao, H., Ghosheh, Y., Gulati, R., Durant, C., Vallejo, J., Saigusa, R., Platts-Mills, T. A. E., Taylor, A. M., Ley, K. & McNamara, C. A., 2021, In: Frontiers in Cardiovascular Medicine. 8, 791028.Research output: Contribution to journal › Article › peer-review |
Autoimmune Regulator (AIRE) Deficiency Does Not Affect Atherosclerosis and CD4 T Cell Immune Tolerance to Apolipoprotein BNettersheim, F. S., Braumann, S., Kobiyama, K., Orecchioni, M., Vassallo, M., Miller, J., Ali, A., Roy, P., Saigusa, R., Wolf, D., Ley, K. & Winkels, H., 2021, In: Frontiers in Cardiovascular Medicine. 8, 812769.Research output: Contribution to journal › Article › peer-review |
Human circulating CD24hi marginal zone B cells produce IgM targeting atherogenic antigens and confer protection from vascular diseasePattarabanjird, T., Nguyen, A. T., McSkimming, C., Dinh, H. Q., Marshall, M. A., Ghosheh, Y., Gulati, R., Durant, C., Vallejo, J., Saigusa, R., Drago, F., Guy, T. V., Premo, K., Taylor, A. M., Paul, S., Kundu, B., Berr, S., Gonen, A., Tsimikas, S. & Miller, Y. & 4 others, , Nov 2023, In: Nature Cardiovascular Research. 2, 11, p. 1003-1014 12 p.Research output: Contribution to journal › Article › peer-review |
Identification of human exTreg cells as CD16+CD56+ cytotoxic CD4+ T cellsFreuchet, A., Roy, P., Armstrong, S. S., Oliaeimotlagh, M., Kumar, S., Orecchioni, M., Ali, A. J., Khan, A., Makings, J., Lyu, Q., Winkels, H., Wang, E., Durant, C., Ghosheh, Y., Gulati, R., Nettersheim, F. & Ley, K., Oct 2023, In: Nature Immunology. 24, 10, p. 1748-1761 14 p.Research output: Contribution to journal › Article › peer-review |
Single cell transcriptomics and TCR reconstruction reveal CD4 T cell response to MHC-II-restricted APOB epitope in human cardiovascular diseaseSaigusa, R., Roy, P., Freuchet, A., Gulati, R., Ghosheh, Y., Armstrong Suthahar, S. S., Durant, C. P., Hanna, D. B., Kiosses, W. B., Orecchioni, M., Wen, L., Wu, R., Kuniholm, M. H., Landay, A. L., Anastos, K., Tien, P. C., Gange, S. J., Kassaye, S., Vallejo, J. & Hedrick, C. C. & 5 others, , May 2022, In: Nature Cardiovascular Research. 1, 5, p. 462-475 14 p.Research output: Contribution to journal › Article › peer-review |
Pairing of single-cell RNA analysis and T cell antigen receptor profiling indicates breakdown of T cell tolerance checkpoints in atherosclerosisWang, Z., Zhang, X., Lu, S., Zhang, C., Ma, Z., Su, R., Li, Y., Sun, T., Li, Y., Hong, M., Deng, X., Rafiee Monjezi, M., Hristov, M., Steffens, S., Santovito, D., Dornmair, K., Ley, K., Weber, C., Mohanta, S. K. & Habenicht, A. J. R. & 1 others, , Mar 2023, In: Nature Cardiovascular Research. 2, 3, p. 290-306 17 p.Research output: Contribution to journal › Article › peer-review |
Integrated single-cell analysis-based classification of vascular mononuclear phagocytes in mouse and human atherosclerosisZernecke, A., Erhard, F., Weinberger, T., Schulz, C., Ley, K., Saliba, A. E. & Cochain, C., Jun 1 2023, In: Cardiovascular Research. 119, 8, p. 1676-1689 14 p.Research output: Contribution to journal › Article › peer-review |
V4C3 MXene Immune Profiling and Modulation of T Cell-Dendritic Cell Function and InteractionFusco, L., Gazzi, A., Shuck, C. E., Orecchioni, M., Ahmed, E. I., Giro, L., Zavan, B., Yilmazer, A., Ley, K., Bedognetti, D., Gogotsi, Y. & Delogu, L. G., Aug 18 2023, In: Small Methods. 7, 8, 2300197.Research output: Contribution to journal › Article › peer-review |
HIV infection and cardiovascular disease have both shared and distinct monocyte gene expression features: Women’s Interagency HIV studyLin, J., Ehinger, E., Hanna, D. B., Qi, Q., Wang, T., Ghosheh, Y., Mueller, K., Anastos, K., Lazar, J. M., Mack, W. J., Tien, P. C., Berman, J. W., Cohen, M. H., Ofotokun, I., Gange, S., Liu, C., Heath, S. L., Tracy, R. P., Hodis, H. N. & Landay, A. L. & 2 others, , May 2023, In: PloS one. 18, 5 May, e0285926.Research output: Contribution to journal › Article › peer-review |
How Lymphatic Endothelial Cells Destabilize Regulatory T CellsLyu, Q. & Ley, K., Feb 1 2023, In: Arteriosclerosis, thrombosis, and vascular biology. 43, 2, p. 215-217 3 p.Research output: Contribution to journal › Editorial › peer-review |
Single-cell transcriptomes and T cell receptors of vaccine-expanded apolipoprotein B-specific T cellsNettersheim, F. S., Ghosheh, Y., Winkels, H., Kobiyama, K., Durant, C., Armstrong, S. S., Brunel, S., Roy, P., Dileepan, T., Jenkins, M. K., Zajonc, D. M. & Ley, K., Jan 5 2023, In: Frontiers in Cardiovascular Medicine. 9, 1076808.Research output: Contribution to journal › Article › peer-review |
Kindlin2 enables EphB/ephrinB bi-directional signaling to support vascular developmentLi, W., Wen, L., Rathod, B., Gingras, A. C., Ley, K. & Lee, H. S., Mar 1 2023, In: Life Science Alliance. 6, 3Research output: Contribution to journal › Article › peer-review |
Deleting interleukin-10 from myeloid cells exacerbates atherosclerosis in Apoe −/− miceOrecchioni, M., Wolf, D., Suryawanshi, V., Winkels, H., Kobiyama, K., Makings, J., Kiosses, W. B. & Ley, K., Jan 2023, In: Cellular and Molecular Life Sciences. 80, 1, 10.Research output: Contribution to journal › Article › peer-review |
Titration of 124 antibodies using CITE-Seq on human PBMCsNettersheim, F. S., Armstrong, S. S., Durant, C., Blanco-Dominguez, R., Roy, P., Orecchioni, M., Suryawanshi, V. & Ley, K., Dec 2022, In: Scientific reports. 12, 1, 20817.Research output: Contribution to journal › Article › peer-review |
Neuroinflammation Plays a Critical Role in Cerebral Cavernous Malformation DiseaseLai, C. C., Nelsen, B., Frias-Anaya, E., Gallego-Gutierrez, H., Orecchioni, M., Herrera, V., Ortiz, E., Sun, H., Mesarwi, O. A., Ley, K., Gongol, B. & Lopez-Ramirez, M. A., Nov 11 2022, In: Circulation research. 131, 11, p. 909-925 17 p.Research output: Contribution to journal › Article › peer-review |
L-selectin: mechanisms and physiological significance of ectodomain cleavage.Smalley, D. M. & Ley, K., 2005, In: Journal of Cellular and Molecular Medicine. 9, 2, p. 255-266 12 p.Research output: Contribution to journal › Review article › peer-review |
Protection from septic peritonitis by rapid neutrophil recruitment through omental high endothelial venulesBuscher, K., Wang, H., Zhang, X., Striewski, P., Wirth, B., Saggu, G., Lütke-Enking, S., Mayadas, T. N., Ley, K., Sorokin, L. & Song, J., Mar 4 2016, In: Nature communications. 7, 10828.Research output: Contribution to journal › Article › peer-review |
Therapeutic inhibition of CXCR2 by Reparixin attenuates acute lung injury in miceZarbock, A., Allegretti, M. & Ley, K., Oct 2008, In: British Journal of Pharmacology. 155, 3, p. 357-364 8 p.Research output: Contribution to journal › Article › peer-review |
Effect of oxygen tension on regulation of arteriolar diameter in skeletal muscle in situPries, A. R., Heide, J., Ley, K., Klotz, K. F. & Gaehtgens, P., 1995, In: Microvascular Research. 49, 3, p. 289-299 11 p.Research output: Contribution to journal › Article › peer-review |
Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of Acute Lung Injury (ALI)Zarbock, A., DiStasi, M. R., Smith, E., Sanders, J. M., Kronke, G., Harry, B. L., Von Vietinghoff, S., Buscher, K., Nadler, J. L. & Ley, K., Oct 1 2009, In: Journal of Immunology. 183, 7, p. 4715-4722 8 p.Research output: Contribution to journal › Article › peer-review |
IL-17A inhibits the expansion of IL-17A-producing T cells in mice through "short-loop" inhibition via IL-17 receptorSmith, E., Stark, M. A., Zarbock, A., Burcin, T. L., Bruce, A. C., Vaswani, D., Foley, P. & Ley, K., Jul 15 2008, In: Journal of Immunology. 181, 2, p. 1357-1364 8 p.Research output: Contribution to journal › Article › peer-review |
Induction of dendritic cell-like phenotype in macrophages during foam cell formationHyung, J. C., Shashkin, P., Gleissner, C. A., Dunson, D., Jain, N., Lee, J. K., Miller, Y. & Ley, K., Apr 24 2007, In: Physiological Genomics. 29, 2, p. 149-160 12 p.Research output: Contribution to journal › Article › peer-review |
Biomedical application of target tracking in clutterGoobic, A. P., Welser, M. E., Acton, S. T. & Ley, K., 2001, In: Conference Record of the Asilomar Conference on Signals, Systems and Computers. 1, p. 88-92 5 p.Research output: Contribution to journal › Conference article › peer-review |
Flow cytometry analysis of immune cells within murine aortasButcher, M. J., Herre, M., Ley, K. & Galkina, E., Jul 2011, In: Journal of Visualized Experiments. 53, e2848.Research output: Contribution to journal › Article › peer-review |
Fucoidin, but not yeast polyphosphomannan PPME, inhibits leukocyte rolling in venules of the rat mesenteryLey, K., Linnemann, G., Meinen, M., Stoolman, L. M. & Gaehtgens, P., Jan 1 1993, In: Blood. 81, 1, p. 177-185 9 p.Research output: Contribution to journal › Article › peer-review |
Identification of novel downstream targets of platelet glycoprotein VI activation by differential proteome analysis: Implications for thrombus formationSchulz, C., Leuschen, N. V., Fröhlich, T., Lorenz, M., Pfeiler, S., Gleissner, C. A., Kremmer, E., Kessler, M., Khandoga, A. G., Engelmann, B., Ley, K., Massberg, S. & Arnold, G. J., May 20 2010, In: Blood. 115, 20, p. 4102-4110 9 p.Research output: Contribution to journal › Article › peer-review |
CXCR6 promotes atherosclerosis by supporting T-cell homing, interferon-γ production, and macrophage accumulation in the aortic wallGalkina, E., Harry, B. L., Ludwig, A., Liehn, E. A., Sanders, J. M., Bruce, A., Weber, C. & Ley, K., Oct 2007, In: Circulation. 116, 16, p. 1801-1811 11 p.Research output: Contribution to journal › Article › peer-review |
MISTICA: Minimum Spanning Tree-Based Coarse Image Alignment for Microscopy Image SequencesRay, N., McArdle, S., Ley, K. & Acton, S. T., Nov 2016, In: IEEE Journal of Biomedical and Health Informatics. 20, 6, p. 1575-1584 10 p.Research output: Contribution to journal › Article › peer-review |
Interleukin-17 signaling in inflammatory, Kupffer cells, and hepatic stellate cells exacerbates liver fibrosis in miceMeng, F., Wang, K., Aoyama, T., Grivennikov, S. I., Paik, Y., Scholten, D., Cong, M., Iwaisako, K., Liu, X., Zhang, M., Sterreicher, C. H., Stickel, F., Ley, K., Brenner, D. A. & Kisseleva, T., Sep 2012, In: Gastroenterology. 143, 3, p. 765-776.e3Research output: Contribution to journal › Article › peer-review |
The trafficking protein JFC1 regulates Rac1-GTP localization at the uropod controlling neutrophil chemotaxis and in vivo migrationRamadass, M., Johnson, J. L., Marki, A., Zhang, J., Wolf, D., Kiosses, W. B., Pestonjamasp, K., Ley, K. & Catz, S. D., Jun 2019, In: Journal of Leukocyte Biology. 105, 6, p. 1209-1224 16 p.Research output: Contribution to journal › Article › peer-review |
Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720Kharel, Y., Lee, S., Snyder, A. H., Sheasley-O'Neill, S. L., Morris, M. A., Setiady, Y., Zhu, R., Zigler, M. A., Burcin, T. L., Ley, K., Tung, K. S. K., Engelhard, V. H., Macdonald, T. L., Pearson-White, S. & Lynch, K. R., Nov 4 2005, In: Journal of Biological Chemistry. 280, 44, p. 36865-36872 8 p.Research output: Contribution to journal › Article › peer-review |
CXC chemokine ligand 4 induces a unique transcriptome in monocyte-derived macrophagesGleissner, C. A., Shaked, I., Little, K. M. & Ley, K., May 1 2010, In: Journal of Immunology. 184, 9, p. 4810-4818 9 p.Research output: Contribution to journal › Article › peer-review |
Sialyltransferase specificity in selectin ligand formationEllies, L. G., Sperandio, M., Underhill, G. H., Yousif, J., Smith, M., Priatel, J. J., Kansas, G. S., Ley, K. & Marth, J. D., Nov 15 2002, In: Blood. 100, 10, p. 3618-3625 8 p.Research output: Contribution to journal › Article › peer-review |
Editorial: Tyrosine sulfation of leukocyte adhesion molecules and chemokine receptors promotes atherosclerosisKoltsova, E. & Ley, K., Nov 2009, In: Arteriosclerosis, thrombosis, and vascular biology. 29, 11, p. 1709-1711 3 p.Research output: Contribution to journal › Editorial › peer-review |
