The mission of the Georgia Cancer Center's Immune Monitoring Shared Resource (IMSR) laboratory is to provide comprehensive immune monitoring services and expertise to assist researchers and clinicians in designing and performing basic and translational research projects.
The main objective of the resource is to provide standardized immune-based assays, to develop, optimize, and validate new technologies, and to support efforts in the areas of human and animal immunology and immunotherapy.
The IMSR works closely with the Flow Cytometry Resource, the Integrated Genomics Shared Resource, and the Tumor Tissue and Serum Repository at the Georgia Cancer Center.
The Georgia Cancer Center's Immune Monitoring team is proud to offer the services below to all in-house and external researchers looking for certain types of assistance with their data and experiments.
Health Sciences Campus
Valentyna Fesenkova, PhD
IMSR Research Manager
Rafal Pacholczyk, PhD
Multi-color flow cytometric analysis allows characterization of the immune phenotypes (e.g., immune cell subsets, activation & differentiation status) of different immune cell populations in human and animal models. We offer custom designed panels based on individual customer needs.
Intracellular staining of transcription factors (e.g., Foxp3) and cytokines to determine simultaneously the phenotype of cells and their cytokine production profile. We also offer several options for in vitro stimulation (e.g., LPS, PMA+Ionomycin, anti-CD3 and CD28 stimulation) of PBMCs for cytokine profiling.
Detection of a single analyte using commercially available standard or competitive ELISA assays
Measurement of soluble proteins (up to 13 per sample) in plasma/serum, supernatants and selected cell lysates using flow cytometry. This technology utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies. (Kits available from Biolegend or BD Biosciences)
The Luminex technology allows for the measurement of up to 100 distinct proteins through a suspension bead array that employs a series of distinct color-coded microspheres to simultaneously detect multiple soluble analytes, from a single serum, plasma, tissue culture supernatant, or other bodily fluid sample. (Kits available from Bio-Rad, Thermo, R&D, Millipore etc)
We offer project based high - throughput sequencing of human T cell receptors (TCRs) from RNA or DNA.
From RNA: simultaneous preparation of barcoded TCRalpha and TCRbeta library (PCR-unbiased, using template-switching RT PCR technology) for Illumina Mi-SEQ PE sequencing
From genomic DNA: preparation of barcoded TCRbeta - targeted library for Illumina Mi-SEQ PE sequencing
Service includes library pooling, MI-SEQ sequencing in collaboration with our Integrated Genomics Resource, QC quantitation, CDR3 clone extraction and annotation (alignment, error correction, V (D) J and CDR3 assignment). We also offer high level TCR analysis including diversity measure, frequency distribution, clone tracking etc.
Please, inquire for mouse TCR repertoire sequencing.
Magnetic-bead based isolation of immune cell subsets (e.g., T cells, B cells and monocytes) from whole blood samples or PBMCs.
Please, inquire about non-human cell isolation
Consultation - Experimental design discussion
Data Analysis - Custom data processing and analysis
Grant/Manuscript Assistance - Assistance in writing sections of your manuscript or grant applications involving services provided by the IMSR
Choudhary, V., Uaratanawong, R., Patel, R.R., Patel, H., Bao, W., Hartney, B., Cohen, E., Chen, X., Zhong, Q., Isales, C.M., et al. (2019). Phosphatidylglycerol Inhibits Toll-Like Receptor–Mediated Inflammation by Danger-Associated Molecular Patterns. J Invest Dermatol 139, 868-877.
Singla, B., Ghoshal, P., Lin, H., Wei, Q., Dong, Z., and Csányi, G. (2018). PKCδ-Mediated Nox2 Activation Promotes Fluid-Phase Pinocytosis of Antigens by Immature Dendritic Cells. Frontiers in Immunology 9, 537-537.
Xiao, W., Klement, J.D., Lu, C., Ibrahim, M.L., and Liu, K. (2018). IFNAR1 Controls Autocrine Type I IFN Regulation of PD-L1 Expression in Myeloid-Derived Suppressor Cells. The Journal of Immunology 201, 264-277.
Sharma, M.D., Rodriguez, P.C., Koehn, B.H., Baban, B., Cui, Y., Guo, G., Shimoda, M., Pacholczyk, R., Shi, H., Lee, E.-J., et al. (2018). Activation of p53 in Immature Myeloid Precursor Cells Controls Differentiation into Ly6c+CD103+ Monocytic Antigen-Presenting Cells in Tumors. Immunity 48, 91-106.e106.
The Georgia Cancer Center's Immune Monitoring Shared Resource values your feedback. Please, take the time to complete this survey to let us know how your experience was and if there are any opportunities for improvement. Thank you for working with us and for sharing your insights.