Microscopy Seminars & Workshops

ibiology image

iBioseminars on Microscopy Topics

iBioseminars on microscopy Topics

 

Fluorescence Microscopy 

Optical Sectioning & Confocal Microscopy

Bioimage Analysis Course

Microscopy Training Websites

adding a fluorophore

(fluorophore selection to predict and prevent crosstalk)

(A) Adding a fluorophore—press the Add fluorophore button and begin typing the name of the fluorophore. Continue to type in the name of the dye or probe to shorten the list or scroll through the list that is displayed. After a dye or probe is selected it is displayed on the plot. Selecting the Ex (excitation), Em (emission) and Display on plot to the right allows the ability to control which spectra data to remove or display on the plot. The number of Fluorophores added to the SpectraViewer will be displayed by a number located to the left of Fluorophores.

 

(B) Additional fluorophores—continue to select additional fluorophores by using the Add fluorophore at the bottom of the right-hand column.

Thermo Fisher SpectraViewer Guide (Fluorophore Selection) 

 

Nikon tutorial

(NIS Elements tutorials and basic microscopy-must request access)

Nikon E-Learning Site

 

Aliasing Plot

     

Huygens Scientific Volume Imaging

(Determine Optimal Sampling)

 

 

 

 

 

Resources