The Transgenic Zebrafish Core Laboratory functions to facilitate use of zebrafish as a vertebrate model system for basic and biomedical science and comparative genomics. Services, equipment, and resources are available to investigators associated with Augusta University or the University System of Georgia on a fee-for-service basis.

Located in CA-3043 (Interdisciplinary Research Building), the Zebrafish Core Laboratory is equipped to maintain ~50,000 adult fish (3000 aquaria) in a temperature and light-controlled environment. A dedicated staff ensures continuity of care, breeding, and quality embryo production. Core Laboratory microscopes, imaging equipment, and computers are available for use by all Augusta University research laboratories.

Services:

  • Consultation
  • Embryo production
  • Transgenics
  • Maintenance of wild-type, transgenic and
    mutant lines
  • Technical assistance and training
  • Pilot projects and feasibility studies

Research Area:

  • Cardio-Oncology
  • Cardiology
  • Oncology
  • Neurology
  • Brain Research
  • Diabetes and Endocrinology
  • Gastroenterology
  • Hematology
  • Immunology
  • Pathology
  • Vision Discovery
  • Behavioral Science
  • Oral Biology
  • Toxicology
  • Drug Discovery

 

Instrumentation

Zebrafish embryos are optically transparent and easily accessible for observation and experimental manipulation. To exploit these features the Zebrafish Core is well equipped with the following instrumentation.

Microscopy

  • Zeiss AxioPlan 2 upright microscope
    40X DIC/water objective
    63X DIC/water objective
    100X DIC/oil objective
  • Leica MZ FLIII stereomicroscope
  • Zeiss Stemmi SV11 stereomicroscope
  • Zeiss AxioVert100 inverted microscope with filters for EGFP, YFP, and CFP

Behavioral Assay

  • Viewpoint zebrabox and zebralab high throughput locomotor activity tracking and analysis package
  • TSE Visiotracker optokinetic behavior tracking and analysis package

Imaging

  • Hammamatsu Orca CCD camera
  • Diagnostics Instruments Spot RT slider camera system
  • Zeiss ZVS video system / Panasonic SVHS time lapse recorder
  • Kodak DCS 420 digital camera

Other Equipment

  • Micropoint pulsed nitrogen laser for heat shock and cell ablation.
  • Sutter P-97 horizontal needle puller
  • BioRad CHEF-DR III Pulsed Field Electrophoresis System

Contact Information

Associate Professor,
Department of Neuroscience and Regnerative Medicine
Department of Cellular Biology and Anatomy

Surendra (Raj) Rajpurohit, PhD

  • Zebrafish Core P.I.

Research Scientist

map-marker icon 1410 Laney Walker Blvd.
Georgia Cancer Center CN3113
Augusta, GA 30912

Media Kit

In the News

Center for Undergraduate Research and Scholarship announces spring fellowship winners

March 9, 2022JagWire

Annabelle O'Keefe studies the impact COVID-19 had on nursing homes.

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Going fishing for the next treatment for a deadly form of brain cancer

July 10, 2019JagWire

One researcher at the Georgia Cancer Center believes a small fish could be key to solving a big problem in the brain.

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Research

Dr. Rajpurohit's Publications  Dr. Rajpurohit's Google Scholar Profile

Additional Resources

ZFIN: The Zebrafish Information Network  Zebrafish International Resource Center  Georgia Research Alliance