The DNRM Microscopy Facility houses two state-of-the-art Nikon A1R MP+ multiphoton/confocal
microscopes, one Nikon C1 confocal microscope, two Nikon inverted fluorescent microscopes,
and one Zeiss upright fluorescent microscope.
Useful links:
Confocal excitation and emission
Resonant Scanners
Intro to Multiphoton Microscopy
Nikon A1R MP+ Multiphoton/Confocal Microscope, System A
- Four high-sensitivity detectors for multiphoton imaging
- 7 visible laser lines (440, 458, 471, 488, 514, 561, 640nm) for confocal imaging
- MaiTai eHP DS ultrafast laser
- Full electrophysiology recording capabilities
- Fast imaging: 30 frames/second at 512x512 resolution, 420 frames/second maximum speed,
high speed piezo-drive focus motor.
- Simultaneous photostimulation and imaging
- Best-in-class two-photon objective (25x, 1.1N.A., 2mm free working distance)
Nikon A1R MP+ Multiphoton/Confocal Microscope, System B
- Four high-sensitivity detectors for multiphoton imaging
- 4 visible laser lines (405, 488, 561, 640nm) for confocal imaging
- MaiTai eHP DS Ultrafast laser
- Fast imaging: 30 frames/second at 512x512 resolution, 420 frames/second maximum speed,
high speed piezo-drive focus motor.
- Simultaneous photostimulation and imaging
- Best-in-class two-photon objective (25x, 1.1N.A., 2mm free working distance)
Nikon C1 confocal
- 3 visible laser lines (488, 543, 640nm) for confocal imaging
- Imaging workstation with deconvolution software
Nikon Eclipse TE-2000e Inverted Fluorescent Microscope
- Temperature controlled stage
- Camera for high speed imaging
Nikon Eclipse TE-300 Inverted Fluorescent Microscope
- Fitted with environmental controlled chamber for live cell imaging
Zeiss Axioplan2 Fluorescent Microscope
- Multi-channel fluorescent imaging
- Color CCD camera
