The Integrated Genomics Shared Resource in the Georgia Cancer Center offer cutting edge technologies for DNA and RNA sequencing as well as spatial transcriptomic.
We offer a wide range of services from sample processing from both fresh and frozen, as well as fixed cells or tissues to library preparation and next-generation sequencing (NGS) for bulk DNA/RNA sequencing and single-cell/nuclei sequencing. For single-cell studies, we are equipped with the Chromium X system from 10X Genomics. Our sequencing capabilities include the Illumina Novaseq6000 and NextSeq 500. We recently introduce single cell spatial transcriptomics with CosMx SMI platform been able to provide support to investigator that are focusing on single cell in tissue in a specific microenvironment. We are also equipped with CytAssit instrument from 10X genomics for VISIUM spatial transcriptomic. For nucleic acid and protein extraction we are equipped with QIAConnect from Qiagen.
For more information please see the Integrated Genomics Additional Info on the Shared Resources page.
Users are responsible for knowing how to run the equipment and what products and sample preparation to use.
All services are booked through iLAB
Integrated Genomics
CN 3162
Martina Zoccheddu
, PhD -
Director
Miao Yu, PhD
Research Manager
(706) 721-5471
The StepOnePlus Real-Time PCR instrument utilizes an easy-to-use interface and program wizards to help guide the researchers through the process of setting up a run.
It runs on a 96-well format, and uses up to 4 colored dyes. The instrument can be used for multiple applications, including:
The service fee is $10 per use for runs on the StepOnePlus Real-Time PCR instrument.
Qiagen |Sample Purification
QIAcube Connect is designed to perform fully automated purification of nucleic acids and proteins in molecular biology applications. The system is intended for use by professional users trained in molecular biological techniques and the operation of QIAcube Connect.
10X Genomics |Single Cell & Spatial Transcriptomic Platform
The Chromium X Series instrument enables seamless scaling from pilot experiments through million-cell studies, and offers compatibility with an expanding range of Chromium Single Cell applications.
Nanostring | Single cell Spatial Transcriptomic Platform
CosMx SMI is the first high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution.
illumina | Next Generation Sequencing
The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. Its tunable output generates up to 6 Tb and 20B single reads in dual flow cell mode with streamlined workflows.
Martina Zoccheddu, PhD
Miao Yu, PhD
Sahiti Sri Manam, PhD