A fluorimeter measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength. All fluorescent objects have its own fingerprint, meaning that it excites and emits predictable types of light. Fluorescent samples produce an emission spectrum that is measured through a monochromator connected to a photomultiplier detector similar to spectrophotometer. After exciting a sample, a Fluorometer allow us to obtain and measure various attributes of that sample’s fluorescence, including its intensity, spectral wavelengths and lifetime. The fluorescence of a sample can be an indirect probe of the sample's environment, describing the solvent-solute interactions, inter- and intramolecular interactions, and so on. Fluorescence can be used for both qualitative and quantitative analysis of samples. The core facility hosts a HORIBA PTI fluorimeter.
Flourimeter