Transgenic DNA microinjection-only service

  • This service includes one microinjection session using C57BL/6J embryos.
  • DNA template will be provided by the users.
  • For each microinjection session, we will inject ≥150 C57BL/6J embryos, perform embryo transfers to pseudopregnant females.
  • Tail tip biopsies will be provided to the investigator's laboratory for genotyping. Mouse pups will be transferred to the investigator at weaning.

 dsDNA synthesis and purification for microinjection

  • DNA template will be synthesized and cloned into plasmid.
  • Mid- or large-preparation of plasmid DNA will be performed and dsDNA fragment will be isolated and purified for microinjection.