Singh Laboratory
Dr. Nagendra Singh
Professor, Department of Biochemistry & Molecular Biology
Dr. Singh’s laboratory studies molecular and cellular mechanisms of underlying immune responses against pathogens and cancers. We utilize a variety of immunological, epigenetic, molecular, biochemical, and cellular techniques to study the behavior of immune cells during pathological conditions.
One of the ongoing projects in the laboratory involve studying the development, survival, and function of plasma cells. Plasma cells are factory for producing antibodies molecule that neutralize the invading pathogens. Understanding of these mechanisms is critical in designing improved vaccines against pathogens.
His other research interest includes how T cells are activated and generate immune responses and help other immune cells to successfully eliminate invading pathogens and cancers. Under this project, he is studying how T cells become exhausted or toothless during chronic infection and cancers.
Previously, he discovered the mechanism underlying dietary fiber mediated induction of anti-inflammatory mechanisms in intestine such as Treg cells leading to suppression of intestinal inflammation and cancers.
His research is funded by extramural sources such as NIH/NIAID.
Jump to: Selected Publications
Pure Publications
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UFL1 promotes survival and function of virtual memory CD8 T cellsBhatt, B., Kumar, K., Shi, H., Ganesan, D., Anazodo, F., Rathakrishnan, A., Zhu, H., Wanna, A., Jiang, C., Jayavelu, T., Lokeshwar, V. B., Pacholczyk, R., Munn, D. H., Sheridan, B. S., Moskophidis, D., Li, H. & Singh, N., Mar 1 2025, In: Journal of immunology (Baltimore, Md. : 1950). 214, 3, p. 446-459 14 p.Research output: Contribution to journal › Article › peer-review |
Simple virus-free mouse models of COVID-19 pathologies and oral therapeutic interventionZhu, H., Sharma, A. K., Aguilar, K., Boghani, F., Sarcan, S., George, M., Ramesh, J., Van Der Eerden, J., Panda, C. S., Lopez, A., Zhi, W., Bollag, R., Patel, N., Klein, K., White, J., Thangaraju, M., Lokeshwar, B. L., Singh, N. & Lokeshwar, V. B., Mar 15 2024, In: iScience. 27, 3, 109191.Research output: Contribution to journal › Article › peer-review |
Ufmylation of UFBP1 Is Dispensable for Endoplasmic Reticulum Stress Response, Embryonic Development, and Cardiac and Intestinal HomeostasisTandra, V., Anderson, T., Ayala, J. D., Weintraub, N. L., Singh, N., Li, H. & Li, J., Aug 2023, In: Cells. 12, 15, 1923.Research output: Contribution to journal › Article › peer-review |
In Vitro Stimulation of IRE1α/XBP1-Deficient B Cells with LPSZhu, H., Jiang, C., Kaufman, R. J., Li, H. & Singh, N., 2022, Methods in Molecular Biology. Humana Press Inc., p. 221-231 11 p. (Methods in Molecular Biology; vol. 2378).Research output: Chapter in Book/Report/Conference proceeding › Chapter |
RAD51AP1 loss attenuates colorectal cancer stem cell renewal and sensitizes to chemotherapyBridges, A. E., Ramachandran, S., Tamizhmani, K., Parwal, U., Lester, A., Rajpurohit, P., Morera, D. S., Hasanali, S. L., Arjunan, P., Jedeja, R. N., Patel, N., Martin, P. M., Korkaya, H., Singh, N., Manicassamy, S., Prasad, P. D., Lokeshwar, V. B., Lokeshwar, B. L., Ganapathy, V. & Thangaraju, M., Sep 2021, In: Molecular Cancer Research. 19, 9, p. 1486-1497 12 p.Research output: Contribution to journal › Article › peer-review |
Cdk5rap3 is essential for intestinal Paneth cell development and maintenanceQuintero, M., Liu, S., Xia, Y., Huang, Y., Zou, Y., Li, G., Hu, L., Singh, N., Blumberg, R., Cai, Y., Xu, H. & Li, H., Jan 2021, In: Cell Death and Disease. 12, 1, 131.Research output: Contribution to journal › Article › peer-review |
Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunityYang, W., Yu, T., Huang, X., Bilotta, A. J., Xu, L., Lu, Y., Sun, J., Pan, F., Zhou, J., Zhang, W., Yao, S., Maynard, C. L., Singh, N., Dann, S. M., Liu, Z. & Cong, Y., Dec 1 2020, In: Nature communications. 11, 1, 4457.Research output: Contribution to journal › Article › peer-review |
Niacin protects against abdominal aortic aneurysm formation via GPR109A independent mechanisms: Role of NAD1/nicotinamideHorimatsu, T., Blomkalns, A. L., Ogbi, M., Moses, M., Kim, D., Patel, S., Gilreath, N., Reid, L., Benson, T. W., Pye, J., Ahmadieh, S., Thompson, A., Robbins, N., Mann, A., Edgell, A., Benjamin, S., Stansfield, B. K., Huo, Y., Fulton, D. J. & Agarwal, G. & 4 others, , Dec 1 2020, In: Cardiovascular Research. 116, 14, p. 2226-2238 13 p.Research output: Contribution to journal › Article › peer-review |
RAD51AP1 deficiency reduces tumor growth by targeting stem cell self-renewalBridges, A. E., Ramachandran, S., Pathania, R., Parwal, U., Lester, A., Rajpurohit, P., Morera, D. S., Patel, N., Singh, N., Korkaya, H., Manicassamy, S., Prasad, P. D., Lokeshwar, V. B., Lokeshwar, B. L., Ganapathy, V. & Thangaraju, M., Sep 15 2020, In: Cancer Research. 80, 18, p. 3855-3866 12 p.Research output: Contribution to journal › Article › peer-review |
Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid CellsBhatt, B., Zhu, H., Patel, N. & Singh, N., 2020, Methods in Molecular Biology. Humana Press Inc., p. 93-98 6 p. (Methods in Molecular Biology; vol. 2121).Research output: Chapter in Book/Report/Conference proceeding › Chapter |
Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammationCai, Y., Zhu, G., Liu, S., Pan, Z., Quintero, M., Poole, C. J., Lu, C., Zhu, H., Islam, B., Van Riggelen, J., Browning, D. D., Liu, K., Blumberg, R., Singh, N. & Li, H., Dec 1 2019, In: Cell Discovery. 5, 1, 7.Research output: Contribution to journal › Article › peer-review |
Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPRZhu, H., Bhatt, B., Sivaprakasam, S., Cai, Y., Liu, S., Kodeboyina, S. K., Patel, N., Savage, N. M., Sharma, A. K., Kaufman, R. J., Li, H. & Singh, N., Dec 1 2019, In: Nature communications. 10, 1, 1084.Research output: Contribution to journal › Article › peer-review |
A chemokine/chemokine receptor signature potentially predicts clinical outcome in colorectal cancer patientsMitchell, A., Hasanali, S. L., Morera, D. S., Baskar, R., Wang, X., Khan, R., Talukder, A., Li, C. S., Manoharan, M., Jordan, A. R., Wang, J., Bollag, R. J., Singh, N., Albo, D., Ghosh, S. & Lokeshwar, V. B., Nov 1 2019, In: Cancer Biomarkers. 26, 3, p. 291-301 11 p.Research output: Contribution to journal › Article › peer-review |
Systemic antibiotic therapy reduces circulating inflammatory dendritic cells and Treg–Th17 plasticity in periodontitisRajendran, M., Looney, S., Singh, N., Elashiry, M., Meghil, M. M., El-Awady, A. R., Tawfik, O., Susin, C., Arce Munoz, R. M. & Cutler, C. W., May 1 2019, In: Journal of Immunology. 202, 9, p. 2690-2699 10 p.Research output: Contribution to journal › Article › peer-review |
Deficiency of metabolite sensing receptor HCA2 impairs the salutary effect of niacin in hemorrhagic shockSubramani, K., Chu, X., Warren, M., Lee, M., Lu, S., Singh, N. & Raju, R. P., Mar 1 2019, In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 1865, 3, p. 688-695 8 p.Research output: Contribution to journal › Article › peer-review |
Loss of GPR109A/HCAR2 induces aging-associated hepatic steatosisJadeja, R. N., Jones, M. A., Fromal, O., Powell, F. L., Khurana, S., Singh, N. & Martin, P. M., Jan 1 2019, In: Aging. 11, 2, p. 386-400 15 p.Research output: Contribution to journal › Article › peer-review |
Oral Pathobiont Activates Anti-Apoptotic Pathway, Promoting both Immune Suppression and Oncogenic Cell ProliferationArjunan, P., Meghil, M. M., Pi, W., Xu, J., Lang, L., El-Awady, A., Sullivan, W., Rajendran, M., Rabelo, M. S., Wang, T., Tawfik, O. K., Kunde-Ramamoorthy, G., Singh, N., Thangaraju, M., Susin, C., Teng, Y., Arce Munoz, R. M. & Cutler, C. W., Dec 1 2018, In: Scientific reports. 8, 1, 16607.Research output: Contribution to journal › Article › peer-review |
Gpr109a limits microbiota-induced IL-23 production to constrain ILC3-mediated colonic inflammationBhatt, B., Zeng, P., Zhu, H., Sivaprakasam, S., Li, S., Xiao, H., Dong, L., Shiao, S.-Y. P., Kolhe, R. B., Li, H., Levy Bercowski, D., Ganapathy, V., Singh, N. & Patel, N., Apr 15 2018, In: Journal of Immunology. 200, 8, p. 2905-2914 10 p.Research output: Contribution to journal › Article › peer-review |
Cyclic-GMP-elevating agents suppress polyposis in Apc Min mice by targeting the preneoplastic epitheliumSharman, S. K., Islam, B. N., Hou, Y., Singh, N., Berger, F. G., Sridhar, S., Yoo, W. & Browning, D. D., Feb 2018, In: Cancer Prevention Research. 11, 2, p. 81-91 11 p.Research output: Contribution to journal › Article › peer-review |
Carbidopa, a drug in use for management of Parkinson disease inhibits T cell activation and autoimmunityZhu, H., Lemos, H., Bhatt, B., Islam, B. N., Singh, A., Gurav, A., Huang, L., Browning, D. D., Mellor, A., Fulzele, S. & Singh, N., Sep 2017, In: PloS one. 12, 9, e0183484.Research output: Contribution to journal › Article › peer-review |
The Relationship Between Probiotics and Dietary Fiber Consumption and Cardiovascular HealthPrasad, P. D., Gurav, A., Zhu, H., Martin, P. M., Vijay-Kumar, M. & Singh, N., Jul 13 2017, Dietary Fiber for the Prevention of Cardiovascular Disease: Fiber's Interaction between Gut Micoflora, Sugar Metabolism, Weight Control and Cardiovascular Health. Elsevier Inc., p. 73-90 18 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter |
Sildenafil normalizes bowel transit in preclinical models of constipationSharman, S. K., Islam, B. N., Hou, Y., Usry, M., Bridges, A., Singh, N., Sridhar, S., Rao, S. & Browning, D. D., Apr 2017, In: PloS one. 12, 4, e0176673.Research output: Contribution to journal › Article › peer-review |
JAK-STAT-mediated chronic inflammation impairs cytotoxic T lymphocyte activation to decrease anti-PD-1 immunotherapy efficacy in pancreatic cancerLu, C., Talukder, A., Savage, N. M., Singh, N. & Liu, K., Mar 4 2017, In: OncoImmunology. 6, 3, e1291106.Research output: Contribution to journal › Article › peer-review |
cGMP Signaling Increases Antioxidant Gene Expression by Activating Forkhead Box O3A in the Colon EpitheliumWang, R., Islam, B. N., Bridges, A., Sharman, S. K., Hu, M., Hou, Y., Somanath, P. R., Venable, L., Singh, N., Kim, S., Sridhar, S., Hofmann, F. & Browning, D. D., Feb 1 2017, In: American Journal of Pathology. 187, 2, p. 377-389 13 p.Research output: Contribution to journal › Article › peer-review |
The impact of antibiotic usage on the efficacy of chemoimmunotherapy is contingent on the source of tumorreactive T cellsKuczma, M. P., Ding, Z.-C., Li, T., Habtetsion, T., Chen, T., Hao, Z., Bryan, L. J., Singh, N., Kochenderfer, J. N. & Zhou, G., 2017, In: Oncotarget. 8, 67, p. 111931-111942 12 p.Research output: Contribution to journal › Article › peer-review |
Sildenafil suppresses inflammation-driven colorectal cancer in miceIslam, B. N., Sharman, S. K., Hou, Y., Bridges, A. E., Singh, N., Kim, S., Kolhe, R. B., Trillo-Tinoco, J., Rodriguez, P. C., Berger, F. G., Sridhar, S. & Browning, D. D., 2017, In: Cancer Prevention Research. 10, 7, p. 377-388 12 p.Research output: Contribution to journal › Article › peer-review |
Benefits of short-chain fatty acids and their receptors in inflammation and carcinogenesisSivaprakasam, S., Prasad, P. D. & Singh, N., Aug 1 2016, In: Pharmacology and Therapeutics. 164, p. 144-151 8 p.Research output: Contribution to journal › Review article › peer-review |
Essential role of Ufm1 conjugation in the hematopoietic systemCai, Y., Singh, N. & Li, H., Jun 1 2016, In: Experimental Hematology. 44, 6, p. 442-446 5 p.Research output: Contribution to journal › Review article › peer-review |
An essential role of Ffar2 (Gpr43) in dietary fibre-mediated promotion of healthy composition of gut microbiota and suppression of intestinal carcinogenesisSivaprakasam, S., Gurav, A., Paschall, A. V., Coe, G. L., Chaudhary, K., Cai, Y., Kolhe, R., Martin, P., Browning, D., Huang, L., Shi, H., Sifuentes, H., Vijay-Kumar, M., Thompson, S. A., Munn, D. H., Mellor, A., McGaha, T. L., Shiao, P., Cutler, C. W. & Liu, K. & 3 others, , 2016, In: Oncogenesis. 5, 6, e238.Research output: Contribution to journal › Article › peer-review |
RCAD/Ufl1, a Ufm1 E3 ligase, is essential for hematopoietic stem cell function and murine hematopoiesisZhang, M., Zhu, X., Zhang, Y., Cai, Y., Chen, J., Sivaprakasam, S., Gurav, A., Pi, W., Makala, L. H., Wu, J., Pace, B. S., Tuan Lo, D., Ganapathy, V., Singh, N. & Li, H., Dec 1 2015, In: Cell Death and Differentiation. 22, 12, p. 1922-1934 13 p.Research output: Contribution to journal › Article › peer-review |
UFBP1, a Key Component of the Ufm1 Conjugation System, Is Essential for Ufmylation-Mediated Regulation of Erythroid DevelopmentCai, Y., Pi, W., Sivaprakasam, S., Zhu, X., Zhang, M., Chen, J., Makala, L. H., Lu, C., Wu, J., Teng, Y., Pace, B. S., Tuan Lo, D., Singh, N. & Li, H., Nov 2015, In: PLoS Genetics. 11, 11, e1005643.Research output: Contribution to journal › Article › peer-review |
Slc5a8, a Na+-coupled high-affinity transporter for short-chain fatty acids, is a conditional tumour suppressor in colon that protects against colitis and colon cancer under low-fibre dietary conditionsGurav, A., Sivaprakasam, S., Bhutia, Y. D., Boettger, T., Singh, N. & Ganapathy, V., Jul 15 2015, In: Biochemical Journal. 469, 2, p. 267-278 12 p.Research output: Contribution to journal › Article › peer-review |
Microbiota-Dependent Hepatic Lipogenesis Mediated by Stearoyl CoA Desaturase 1 (SCD1) Promotes Metabolic Syndrome in TLR5-Deficient MiceSingh, V., Chassaing, B., Zhang, L., San Yeoh, B., Xiao, X., Kumar, M., Baker, M. T., Cai, J., Walker, R., Borkowski, K., Harvatine, K. J., Singh, N., Shearer, G. C., Ntambi, J. M., Joe, B., Patterson, A. D., Gewirtz, A. T. & Vijay-Kumar, M., 2015, In: Cell Metabolism. 22, 6, p. 983-996 14 p.Research output: Contribution to journal › Article › peer-review |
Monomethylfumarate induces γ-globin expression and fetal hemoglobin production in cultured human retinal pigment epithelial (RPE) and erythroid cells, and in intact retina.Promsote, W., Makala, L., Li, B., Smith, S. B., Singh, N., Ganapathy, V., Pace, B. S. & Martin, P. M., Aug 2014, In: Investigative ophthalmology & visual science. 55, 8, p. 5382-5393 12 p.Research output: Contribution to journal › Article › peer-review |
Butyrate-Mediated Protection Against Colonic Inflammation And Colon Carcinogenesis: Role Of Butyrate Transporters And Butyrate ReceptorsVadivel, G., Prasad, P. D., Thangaraju, M., Martin, P. M. & Singh, N., Apr 1 2014, Butyrate: Food Sources, Functions and Health Benefits. Nova Science Publishers, Inc., p. 157-176 20 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter |
The plasma membrane transporter SLC5A8 suppresses tumour progression through depletion of survivin without involving its transport functionCoothankandaswamy, V., Elangovan, S., Singh, N., Prasad, P. D., Thangaraju, M. & Ganapathy, V., Feb 15 2013, In: Biochemical Journal. 450, 1, p. 169-178 10 p.Research output: Contribution to journal › Article › peer-review |
Butyrate suppresses colonic inflammation through HDAC1-dependent fas upregulation and fas-mediated apoptosis of T cellsZimmerman, M. A., Singh, N., Martin, P. M., Thangaraju, M., Ganapathy, V., Maghsoodpour, A., Shi, H., Robertson, K. D., Munn, D. H. & Liu, K., Jun 15 2012, In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 302, 12, p. G1405-G1415Research output: Contribution to journal › Article › peer-review |
SLC6A14 (ATB 0,+) protein, a highly concentrative and broad specific amino acid transporter, is a novel and effective drug target for treatment of estrogen receptor-positive breast cancerKarunakaran, S., Ramachandran, S., Coothankandaswamy, V., Elangovan, S., Babu, E., Periyasamy Thandavan, S., Gurav, A., Gnana-Prakasam, J. P., Singh, N., Schoenlein, P. V., Prasad, P. D., Thangaraju, M. & Ganapathy, V., Sep 9 2011, In: Journal of Biological Chemistry. 286, 36, p. 31830-31838 9 p.Research output: Contribution to journal › Article › peer-review |
IFN-γ upregulates Survivin and ifi202 expression to induce survival and proliferation of tumor-specific T cellsZimmerman, M., Yang, D., Hu, X., Liu, F., Singh, N., Browning, D., Ganapathy, V., Chandler, P., Choubey, D., Abrams, S. I. & Liu, K., 2010, In: PloS one. 5, 11, e14076.Research output: Contribution to journal › Article › peer-review |
Suberoylanilide hydroxamic acid (SAHA) induces growth arrest and apoptosis in pituitary adenoma cellsSangeetha, S. R., Singh, N., Vender, J. R. & Dhandapani, K. M., Jun 2009, In: Endocrine. 35, 3, p. 389-396 8 p.Research output: Contribution to journal › Article › peer-review |